Spacefill model of A-234

Novichok

The notorious nerve agent

Paul May
Bristol University

Molecule of the Month August 2018
Also available: JSMol version.

Scientists in hazchem suits in Salisbury

Wasn’t that the nerve agent used recently to try to assassinate that Russian spy in Salisbury?

Yes, it’s believed so. The victim was Sergei Skripal, who was a former GRU Russian military officer and British spy who acted as a double agent for MI6 during the 1990s-2000s, before being arrested and sent to a prison colony for treason. As part of a spy-swap arrangement, Sergei moved to the UK in 2010 and became a British citizen. It has been suggested that he became a target for the Russian secret services possibly as a warning to other potential spies.

Sergei Skripal Yulia Skripal
Sergei Skripal, before the attack. Yulia Skripal, giving a TV interview after surviving the attack.
The scar on her neck is from a tracheotomy used to help her breath during her treatment.

How was the attack done?

In March 2018, he and his daughter, Yulia (who was visiting him at the time), were found unconscious on a park bench in Salisbury, UK, near where Sergei lived. They were rushed to the local hospital, where their unusual symptoms raised alarms, and tests showed that they had been in contact with a secret nerve agent called ‘Novichok’. Subsequent tests found high concentrations of Novichok on the front-door handle of Yuri’s house, with the implication that it had been placed there deliberately in an attempt to contaminate him. Since Novichok is so deadly, this was effectively an assassination attempt, with the daughter being an innocent victim. Luckily, immediate treatment at the local hospital saved both their lives, although it took 3 months for them both to recover – and even then there remains the risk of some permanent nerve damage. But the story didn’t end there…

Specialists in hazmat suits inspecting the forensic covering the bench in Salisbury where the Skripals were found
Specialists in hazmat suits inspecting the forensic covering the bench in Salisbury
where the Skripals were found after the nerve agent attack in March 2018

What happened?

Four months later, 2 more people, Dawn Sturgess and Charlie Rowley, fell ill with identical symptoms in Amesbury, a small town only 7 miles from Salisbury. They were later confirmed to have high concentrations of Novichok on their hands. Neither of these 2 new victims had any apparent link to Russia, and it turns out that Charlie had found a discarded perfume bottle in a recycling bin in Salisbury. Charlie had brought it home, and Dawn then sprayed the contents on her hands, thinking it was a well-known branded perfume. Instead of perfume, it was described as containing an oily liquid which didn't smell of anything much. Charlie got some on his hands too, but crucially, he washed his hands immediately. Dawn did not, and within 15 mins was feeling ill. Although they both were rushed to hospital, sadly, Dawn Sturgess later died, turning an already complicated story into a murder investigation.

But that was 4 months after the first attack...?

Well, the Novichok reagents are reported to have a potency lifetime of up to a year, especially if sealed inside a container. The fact that there might be more discarded bottles of still-active Novichok lying around the streets and parks of Salisbury led to widespread alarm amongst the local population, and accusations from the British Home Secretary, Sajid Javid, that the Russian state was using Britain as a “dumping ground for poison”.

Newspaper reports of the diplomatic expulsions in March 2018This must have all caused a massive political argument!

You could say that! Once it was confirmed that Novichok was to blame, the British government accused Russia of attempted assassination of a British citizen. They announced a series of punishments against Russia, including the expulsion of 23 diplomats. In a show of solidarity with the UK, a large number of countries and other organisations expelled a total of more than 150 Russian diplomats, in what the BBC reported was "the largest collective expulsion of Russian intelligence officers in history”.

What did the Russian government say?

Russia claimed to be innocent, and declared the story to be a fabrication and a "grotesque provocation rudely staged by the British and U.S. intelligence agencies" to undermine Russia. They also expelled 23 British diplomats in a tit-for-tat response.

So what actually is Novichok?

Novichok means ‘newcomer’ in Russian, and was a new type of nerve agent developed secretly in Soviet Russia as a chemical warfare agent in the 1970-1990s.

Don’t the Russians have enough nerve agents already?

Ah, the problem with all the older nerve agents like VX and sarin is that they are well known and quite easy to detect. The Novichok agents were secret and designed to be very difficult to detect, so they could be slipped into foreign countries without being discovered. Also, they were made to be so toxic they would defeat the standard NATO hazmat suits of the time. They were also made from unusual chemical reagents – different from the ones used to make the older generation of nerve agents.

Why?

The purchase and manufacture of all chemicals that can be used to make dangerous or illicit products, such as warfare agents, deadly poisons, or drugs (such as heroin, cocaine), are closely monitored by the police or intelligence agencies of many countries. So being able to make a nerve agent from chemicals which were not on any watch-list and therefore didn’t raise suspicions was a bonus. Another advantage was that the Novichok agents were relatively safe to handle compared to most other nerve agents.

Why was that?

This was because they were binary munitions, in which two non-toxic (or less-toxic) precursor reagents had to be mixed to produce the active agent. This could be done immediately before use, in, say, a syringe, meaning the assassin was far less likely to poison themselves in the preparations for the ‘mission’.

You keep saying ‘Novichok agents’, plural?

It’s believed many Novichok agents were developed, all with different structures and characteristics. The Novichok classification refers to the weapon in its binary form (e.g. Novichok-5 or Novichok-7), whereas once synthesised, the agent is known simply by a code number such as A-232. The first of the series to be made was actually part of the ‘V’ series of chemical warfare agents, of which the best known is VX (MOTM July 2001). This new gas, codenamed VR, was actually a binary munition, so became the first of the Novichok series.

VR
VR- the first of the Novichok agents.

Vil Mirzayanov in 2018If they are so secret, how do we know about them?

Almost everything we know is due to a Russian defector named Vil Mirzayanov (photo, right). He was an analytical chemist who worked for the Russian State Research Institute of Organic Chemistry and Technology - actually a notorious chemical weapons lab. He didn’t make nerve agents there, but instead his job was to develop methods to detect nerve agents that had been created in the various Soviet chemical weapons facilities in order to monitor the environment around the secret labs for any traces of the agents that might reveal the labs’ true activities to foreign intelligence services. In the 1980s, his analysis showed that the air and water around one of these facilities was being poisoned by leaks of these nerve agents, and threatening the health of the local population. To stop this, he revealed details of the Soviet chemical weapons program to the newspaper Moscow News in 1992. He was immediately arrested for revealing state secrets, and sent to a penal colony. But eventually the case against him was dropped and in 1995 he emigrated to America and wrote a book called “State Secrets: An Insider’s Chronicle of the Russian Chemical Weapons Program”. In this he gave details of the whole Novichok programme.

The general formula of a Novichok
The general formula of a Novichok.
R = alkyl, alkoxy, alkylamino, or fluorine
and X = a halogen (F, Cl, Br) or CN.

Despite this, it’s still not known how many nerve agents or their binary Novichok counterparts were actually developed, but it could be as many as several hundred. However, we do know something about their structures. They nearly all are organophosphorus compounds, with various sidegroups attached to change the molecule’s potency or lifetime. These are either in the form of phosphates, or monofluorinated phosphates, or modified versions where the P=O groups are changed to P=Se or P=S.

Often, one of the sidegroups used is phosgene oxime, which is itself a ‘nettle agent’, i.e. it causes chemical burns on exposed skin, as well as rashes and intense itching.

phosgene oxime
Phosgene oxime

In his book, Mirzayanov explains that one range of compounds was developed based on the phosphorus-oxygen-fluorine core of older nerve agents such as sarin and soman. The O-alkyl group in these compounds was substituted with an amidine group (RC(=NR)NR2) to create a molecule they called A-230. This was 5-8 times more poisonous than VX, and was later adopted as a chemical weapon by the Soviet Army. By changing the sidegroup to a methoxy they then produced A-232 (which was just as toxic but more volatile), and to an ethoxy to make A-234, which was an oily liquid. Once binary formulations were invented to make these compounds at will, the Novichoks were born.

Which was the one used in Salisbury?

It is believed that the Novichok codenamed A234 was the one used, but it not yet known (or at least it hasn't been revealed) whether the 'perfume' bottle was the container for the previously made Novichok which was somehow smuggled into the country, or the mixing bottle where the two binary counterparts were combined.

A-230 A-232 A-234
A-230 A-232
The binary analogue of this is known as Novichok-5.
A-234
The binary analogue of this is known as Novichok-7.

Some of the Novichok agents, the main difference between them is the group bonded to the P, which is either a methyl, methoxy or ethoxy group.

On examining the 'perfume' bottle, the police found it had been modified with a special spray nozzle to allow the nerve agent to be sprayed more safely away from the user's hands. They believe this bottle was smuggled through customs at Gatwick airport when the assassins entered the country. After the attack, they then simply discarded it into a recycling bin in Salisbury, still half-full of Novichok, where Charlie Rowley discovered it.

You say 'they' - are the attackers known?

On 5th September 2018 the gvernment revealed that the police investigation had come up with two prime suspects, who had been seen on CCTV around the area of the Skripal's house. The 2 suspects entered the UK on Russian passports using the names Alexander Petrov and Ruslan Boshirov, had stayed in a London hotel for two days, visited Salisbury briefly, and then returned to Moscow. Minute traces of Novichok were also found in the London hotel where they had stayed. The UK Prime Minister, Teresa May, said the suspects are thought to be officers from Russia's military intelligence service the GRU, and that this showed that the poisoning was "not a rogue operation" and was "almost certainly" approved at a senior level of the Russian state.

The two suspects
The two suspects, Alexander Petrov and Ruslan Boshirov, caught on CCTV in Salisbury before the attack.

The modified perfume bottle
The modified bottle, disguised as a bottle of Nina Ricci Premier Jour perfume.
Photo: Metropolitan Police.

How does Novichok work?

In the nervous system, the molecule acetylcholine is needed to allow nerve impulses to pass from a neuron into a muscle, activating it. But after it has done its job, acetylcholine is then broken down by an enzyme called acetylcholinesterase. Without this breakdown process, the muscles contract continuously or randomly or spasm uncontrollably. The Novichok agents inhibit acetylcholinesterase, so acetylcholine concentrations then increase leading to involuntary contraction or spasm of all the muscles in the body. This eventually causes respiratory and cardiac arrest as the muscles in the heart and diaphragm no longer function properly. Copious fluids also fill the victim's lungs, who eventually dies from either suffocation or heart failure.

PralidoximeNasty! How is it treated?

The most common treatment is to give the victim atropine, which blocks the acetylcholine receptors and switches off the signal to the overstimulated muscle cells. This is not without risk, however, as atropine is poisonous in high doses, and the dose required to counteract Novichok is close to the lethal amount. Another drug, pralidoxime chloride, is also used to reactivate acetylcholinesterase and relieve the respiratory muscle paralysis, allowing the victim to breathe. During all this treatment, which could take many weeks in intensive care, the victim also has to have their lungs continuously drained of the secretions which would otherwise suffocate them.

Even so, nerve agents need to be countered fast, by speedy administration of these various treatments. The reason is that the agent forms a permanent linkage to the active site of the acetylcholinesterase (this is known as ‘ageing’), after which the antidote will have no effect. The only hope is that the victim has not had too big a dose of the nerve agent, so they can survive long enough for the body to be able to regenerate the acetylcholinesterase naturally over some weeks. This is what seems to have happened to the Skripals.

What are the long-term implications of this affair?

It's difficult to predict. Even now that the assassins have been identified, it's doubtful whether they would ever be extradited to the UK to stand trial. Similarly, it's unlikely that definitive proof will ever be found that the Russian government sanctioned the 'hit'. But it remains a worry to Western governments that everything points to the fact that Russia had no concerns about using a chemical warfare agent in a foreign country against a foreign citizen, without regard for the implications or safety of the rest of the population. It took probably only a few millilitres of Novichok to render large parts of the town of Salisbury, and later Amesbury, off-limits for several months while they were decontaminated. And it's believed that around 10 tons of A-234 was manufactured in Russia, which is probably still in storage - somewhere...

Bibliography

Wikipedia:

General

Biological activity

Chemistry

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