Publications
60. L. M. Halo, J. W. Marshall, A. A. Yakasai, Z. Song, C. P. Butts, M. P. Crump, M. Heneghan, A. M. Bailey, T. J. Simpson, C. M. Lazarus, and R. J. Cox,* Authentic Heterologous Expression Of The Tenellin Iterative Polyketide Synthase Non-Ribosomal Peptide Synthetase requires co-expression with an enoyl reductase, ChemBioChem, 2007, in press.
59. P. Beltran-Alvarez, R. J. Cox,* J. Crosby and T. J. Simpson, Dissecting the Component Reactions Catalysed by the Actinorhodin Minimal Polyketide Synthase, Biochemistry, 2007, in press.
58. D. E. Shallcross,* T. G. Harrison, P. J. Wyatt, R. J. Cox, N. C. Norman, D. M. Smith and S. J. Croker, Lessons in effective Practical Chemistry at tertiary level: Case studies from the Bristol ChemLabS Outreach Program, Chemistry Education Research and Practice, 2007, in press.
57. A. M. Bailey, R. J. Cox,* K. Harley, C. M. Lazarus, T. J. Simpson* and E. Skellam, Characterisation of 3-methylorcinaldehyde synthase (MOS) in Acremonium strictum: first observation of a reductive release mechanism during polyketide biosynthesis, Chem. Commun., 2007, 4053-4055. DOI: DOI: 10.1039/b708614h
56. R. J. Cox* and U. Wong, The Chemical Mechanism of -1-deoxyxylulose-5-phosphate reductoisomerase from Escherichia coli, Angewandte Chim. Int Ed. Engl., 2007, 46, 4926-4929. DOI: 10.1002/anie.200700647
55. J. Hothersall, J. Wu, A. S. Rahman, J. A. Shields, J. Haddock, N. Johnson, S. M Cooper, E. Stephens, R. J. Cox, J. Crosby, C. L. Willis, T. J Simpson and C. M. Thomas*, Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by Pseudomonas fluorescens: Pseudomonic acid B biosynthesis precedes pseudomonic acid, J. Biol. Chem., 2007, 282, 15451-15461. DOI:10.1074/jbc.M701490200
54. J. Wu, S. M. Cooper, R. J. Cox, J. Crosby, M. P. Crump, J. Hothersall, T. J. Simpson,* C. M. Thomas and C. L. Willis, Mupirocin H, a novel metabolite resulting from mutation of the HMG-CoA synthase analogue, mupH in Pseudomonas fluorescens, Chem. Commun., 2007, 2040-2042. DOI: 10.1039/b700613f.
53. I. Eleftherianos, S. Boundy, S. A. Joyce, J. W. Marshall, R. J. Cox, T. J. Simpson, D. J. Clarke, R. H. ffrench-Constant and S. E. Reynolds,* An antibiotic produced by an insect-pathogenic bacterium inhibits host defences through phenoloxidase inhibition, Proc. Natl. Acad. Sci. USA, 2007, 104, 2419-2424. DOI: 10.1073/pnas.0610525104
52. R. J. Cox* and A. S. Evitt, Acyl Palladium Species in Synthesis: Single Step Synthesis of a,b-Unsaturated Ketones from Acid Chlorides, Org. Biomol. Chem., 2007, 5, 229-232. DOI: 10.1039/b616582f
51. K. L. Eley, L. M. Halo, Z. Song, H. Powles, R. J. Cox,* A. M. Bailey, C. M. Lazarus and T. J. Simpson, Biosynthesis of the 2-Pyridone Tenellin in the Insect Pathogenic Fungus Beauveria bassiana, ChemBioChem, 2007, 8, 289-297. DOI: 10.1002/cbic.200600398
50. D. O. Rees, N. Bushby, R. J. Cox, J. R. Harding, T. J. Simpson* and C. L. Willis,* Synthesis of 1,2-13C215N.--Homoserine and Incorporation by the PKS-NRPS System of Fusarium moniliforme into the Mycotoxin Fusarin C. ChemBioChem, 2007, 8, 46-50. DOI 10.1002/cbic.200600404
49. Y. Ma, L. H. Smith, R. J. Cox,* P. Beltran-Alvarez, C. J. Arthur and T. J. Simpson, Catalytic Relationships between Type I and Type II Iterative Polyketide Synthases: The Aspergillus parasiticus Norsolorinic Acid Synthase. ChemBioChem, 2006, 7, 1951-1958. DOI 10.1002/cbic.200600341
48. C. J. Arthur, A. E. Szafranska, J. Long, J. Mills, R. J. Cox, S. C. Findlow, T. J. Simpson, M. P. Crump and J. Crosby*, The malonyl transferase activity of type II polyketide synthase acyl carrier proteins. , Chem. Biol., 2006, 13, 587-596. DOI:10.1016/j.chembiol.2006.03.010
47. R. J. Cox,* J. S. Gibson and A. T. Hadfield, Design, Synthesis and Analysis of Inhibitors of Bacterial Aspartate Semi-aldehyde Dehydrogenase , ChemBioChem, 2005, 6, 2255-2260. DOI 10.1002/cbic.200500172
46. S.M. Cooper, W. Laosripaiboon, J. Hothersall, A. K. El-Sayed, C. Winfield, J. Crosby, R. J. Cox, T. J. Simpson and C. M. Thomas,* Shift to Pseudomonic Acid B Production in Pseudomonas fluorescens NCIMB 10856 by Mutation of Mupirocin Tailoring Genes mupO, mupU and mupV , Chem. Biol., 2005, 12, 825-833. DOI:10.1016/j.chembiol.2006.03.010
45. L. Merckl?, A. de Andr?s-G?mez, B. Dick, R. J. Cox* and C. R. A. Godfrey A Fragment-Based Approach to Understanding Inhibition of 1-Deoxy--Xylulose-5-Phosphate Reductoisomerase , ChemBioChem, 2005, 6, 1866-1874. DOI 10.1002/cbic.200500061
44. S. M. Cooper, R. J. Cox, J. Crosby, M. P. Crump, J. Hothersall, W. Laosripaiboon, T. J. Simpson* and C. M. Thomas, Mupirocin W, a Novel Pseudomonic Acid Produced by Targeted Mutation of the Mupirocin Biosynthetic Gene Cluster, Chem. Commun., 2005, 1179-1181. DOI: 10.1039/b414781b
43. R. J. Cox,* D. J. Ritson, T. A. Dane, J. Berge, J. P. H. Charmant and A. Kantacha, Room Temperature Palladium Catalysed Coupling of Acyl Chlorides with Terminal Alkynes, Chem. Commun., 2005, 1037-1039. DOI: 10.1039/b414826f
42. R. J. Cox,* F. Glod, D. Hurley, C. M. Lazarus, T. P. Nicholson, B. A. M. Rudd, T. J. Simpson, B. Wilkinson and Y. Zhang, Rapid Cloning and Expression of a Fungal Polyketide Synthase Gene Involved in Squalestatin Biosynthesis, Chem. Commun., 2004, 20, 2260-2261. DOI: 10.1039/b411973h
41. R. J. Cox*, F. Glod, T. J. Simpson, C. M. Lazarus and Z. Song, Biosynthesis of Fusarin C in Fusarium moniliforme and Fusarium venenatum, ChemBioChem, 2004, 5, 1196-1203. DOI 10.1002/cbic.200400138
40. R. J. Cox,* J. Berge and D. J. Ritson, Indium Mediated Allylation of Glyoxylate Oxime Ethers, Esters and Cyanoformates, Org. Biomol. Chem., 2004, 2, 1921-1933. DOI: 10.1039/b402764g
39. W. Teartasin, C. Limpkin, F. Glod, J. Spencer, R. J. Cox, T. J. Simpson, J. Crosby, M. P. Crump, and A. T. Hadfield*, Expression, Purification and Preliminary X-ray Diffraction Analysis of a Ketoreductase from a Type II Polyketide Synthase, Acta Cryst. Sect. D., 2004, 60, 1137-1138. DOI:10.1107/S0907444904007905
38. L. A. Adams, J. P. H. Charmant, R. J. Cox,* M. Walter and W. G. Whittingham, Efficient Synthesis of Protected Cyclopropyl b-Aspartylphosphates, Org. Biomol. Chem. 2004, 2, 542-553. DOI: 10.1039/b311322a
37. L. A. Adams, V. K. Aggarwal,* R. V. Bonnert, B. Bressel, R. J. Cox,* J. Shepherd, J. de Vicente, M. Walter and W. Whittingham, Diastereoselective Synthesis of Cyclopropane Amino Acids Using Diazo Compounds Generated In Situ, J. Org. Chem., 2003, 68, 9433-9440. DOI 10.1021/jo035060c
36. R. J. Cox,* A. de Andres-Gomez and C. R. A. Godfrey, Rapid and flexible synthesis of 1-deoxy- -xylulose-5-phosphate, the substrate for 1-deoxy- -xylulose-5-phosphate reductoisomerase, Org. Biomol. Chem, 2003, 1, 3173-3177. DOI: 10.1039/b307314a
35. T. P. Nicholson, C. Winfield, J. Westcott, J. Crosby, T. J. Simpson, and R. J. Cox,* First in vitro directed biosynthesis of new compounds by a minimal Type II polyketide synthase: evidence for the mechanism of chain length determination, J. Chem. Soc., Chem. Commun., 2003, 686-687. DOI: 10.1039/b300847a
34. M. A. C. Reed, M. Schweizer, A. E. Szafranska, C. Arthur, T. P. Nicholson, R. J. Cox, J. Crosby,* M. P. Crump and T. J. Simpson, The isolated Acyl Carrier Protein Domain from the Type I Rat Fatty Acid Synthase Shows Considerable Homology and Analogous Biochemical Properties to Type II Acyl Carrier Proteins, Organic and Biomolecular Chemistry, 2003, 1, 463-471. DOI: 10.1039/b208941f
33. L. A. Adams, R. J. Cox,* J. S. Gibson, M. B. Mayo-Mart?n, M. Walter and W. Whittingham, A New Synthesis of Phosphoramidates: Inhibitors of Aspartate Semi-Aldehyde Dehydrogenase, J. Chem. Soc., Chem. Commun., 2002, 2004-2005. DOI: 10.1039/b206199f
32. R. J. Cox,* J. S. Gibson and B. Mayo-Martin, Synthesis and in vitro activity of the first synthetic inhibitors of Aspartate semi-aldehyde dehydrogenase, ChemBioChem, 2002, 3, 864-876. DOI 10.1002/1439-7633(20020902)3
31. R. J. Cox,* J. Crosby, O. Daltrop, F. Glod, M. Jarzabek, T. P. Nicholson, T. J. Simpson, F. Soulas and J. Westcott, Cloning, expression and substrate activity of Streptomyces coelicolor holo-Acyl Carrier Protein synthase, J. Chem. Soc., Perkin Trans 1, 2002, 1644-1649. DOI: 10.1039/b204633b
30. R. J. Cox,* J. Durston, and D. I. Roper, Synthesis and in vitro Enzyme Activity of an Oxa Analogue of Azi-DAP, J. Chem. Soc. Perkin Trans 1., 2002, 1029-1035. DOI: 10.1039/b201727j
29. T. S. Hitchman, A. E. Szafranska, R. J. Cox,* J. Crosby* and T. J. Simpson,* Kinetic and Mechanistic Analysis of the Malonyl-CoA:ACP Transacylase from Streptomyces coelicolor Indicates a Single Catalytically Competent Serine Nucleophile at the Active Site, Biochemistry, 2002, 41(5), 1421-1427. DOI 10.1021/bi012001p
28. C. Arthur, R. J. Cox,* J. Crosby, M. M. Rahman, T. J. Simpson, F. Soulas, R. Spogli, A. E. Szafranska, J. Westcott and C. J. Winfield, Synthesis and Characterisation of Acyl Carrier Protein Bound Polyketide Analogues, ChemBioChem, 2002, 3, 253-257. DOI: 10.1002/1439-7633(20020301)3:2/3<253::AID-CBIC253>3.0.CO;2-7
27. R. J. Cox,* A. Hadfield and M. B. Mayo-Martin, Difluoromethylene analogues of aspartyl phosphate: the first synthetic inhibitors of aspartate semi-aldehyde dehydrogenase, J. Chem. Soc., Chem. Commun, 2001, 1710-1711. DOI: 10.1039/b103988c
26. R. J. Cox* and P. S. H. Wang, Synthesis and in vitro enzyme activity of aza, oxa and thia derivatives of bacterial cell wall biosynthesis intermediates, J. Chem. Soc., Perkin Trans. 1, 2001, 2022-2034. DOI: 10.1039/b105117m
25. R. J. Cox* and P. S. H. Wang, Is N-acetylornithine aminotransferase the real N-succinyl- -diaminopimelate aminotransferase in Escherichia coli and Mycobacterium smegmatis?, J. Chem. Soc., Perkin Trans. 1, 2001, 2006-2008. DOI: 10.1039/b106146c
24. T. P. Nicholson, B. A. M. Rudd, M. Dawson, C. M. Lazarus, T. J. Simpson and R. J. Cox,* Design and utility of oligonucleotide gene probes for fungal polyketide synthases, Chem. Biol., 2001, 8, 157-178. DOI:10.1016/S1074-5521(00)90064-4
23. R. J. Cox,* H. Jenkins, J. A. Schouten, R. A. Stentiford and K. J. Wareing, Synthesis and in vitro enzyme activity of peptide derivatives of bacterial cell wall biosynthesis inhibitors, J. Chem. Soc. Perkin Trans. 1, 2000, 2023-2036. DOI: 10.1039/b002701o
22. C. Bisang, P. F. Long J. Cortes, J. Westcott, J. Crosby, A-L Matharu, R. J. Cox, T. J. Simpson, J. Staunton and P. F. Leadlay,* A chain initiation factor common to both modular and aromatic polyketide synthases, Nature, 1999, 401, 502-505. DOI:10.1038/46829
21. A-L. Matharu, R. J. Cox, J. Crosby, K. J. Byrom and T. J. Simpson,* MCAT is not required for in vitro polyketide synthesis in a minimal actinorhodin polyketide synthase from Streptomyces coelicolor, Chem. Biol. 1998, 5, 699-711. DOI:10.1016/S1074-5521(98)90663-9
20. T. S. Hitchman, J. Crosby, K. J. Byrom, R. J. Cox and T. J. Simpson,* Catalytic self-acylation of Type II polyketide synthase acyl carrier proteins, Chem. Biol. 1998, 5, 35-47. DOI:10.1016/S1074-5521(98)90085-0
19. R. J. Cox,* J. A. Schouten, R. A. Stentiford and K. J. Wareing, Peptide inhibitors of N-Succinyl Diaminopimelic Acid Aminotransferase (DAP-AT): A novel class of antimicrobial compounds, Bioorg. Med. Chem. Lett. 1998, 8, 945-950. DOI:10.1016/S0960-894X(98)00149-8
18. M. C. Moore, R. J. Cox, G. R. Duffin, and D. O'Hagan,* Synthesis and evaluation of a putative acyl tetramic acid intermediate in tenellin biosynthesis in Beauveria bassiana. A new role for tyrosine, Tetrahedron, 1998, 54, 9195-9206. DOI:10.1016/S0040-4020(98)00557-2
17. J. Crosby, K. J. Byrom, T. S. Hitchman, R. J. Cox, M. P. Crump, I. S. C. Findlow, M. Bibb and T. J. Simpson,* Acylation of Streptomyces Type II polyketide synthase acyl carrier proteins, FEBS Lett. 1998, 433, 132-138. DOI:10.1016/S0014-5793(98)00840-0
16. R. J. Cox,* T. S. Hitchman, K. J. Byrom, I. S. C. Findlow, J. A. Tanner, J. Crosby and T. J. Simpson, Post-translational modification of heterologously expressed Streptomyces Type II polyketide synthase acyl carrier proteins, FEBS Lett., 1997, 405, 267-272. DOI:10.1016/S0014-5793(97)00202-0
15. R. J. Cox, W. A. Sherwin, L. K. P. Lam and J. C. Vederas,* Synthesis and evaluation of novel substrates and inhibitors of N-Succinyl--diaminopimelate aminotransferase (DAP-AT) from Escherichia coli, J. Am. Chem. Soc., 1996, 118, 7449-7460. DOI 10.1021/ja960640v
14. R. J. Cox and D. O'Hagan,* Synthesis of isotopically labelled 3-amino-2-phenylpropionic acid and its role as a precursor in the biosynthesis of tenellin and tropic acid, J. Chem. Soc., Perkin Trans. 1, 1991, 2537-2540. DOI: 10.1039/P19910002537
Reviews
13. R. J. Cox*, Polyketides, Proteins and Genes in Fungi: Programmed Nano-Machines Begin to Reveal Their Secrets, Org. Biomol. Chem., 2007, 5, 2010-2026. DOI: 10.1039/b704420h
12. R. J. Cox,* Biosynthesis, Annu. Rep. Prog. Chem., Sect. B: Org. Chem., 2002, 98, 223-251. DOI: 10.1039/b111468a
11. R. J. Cox,* Biosynthesis, Annu. Rep. Prog. Chem., Sect. B: Org. Chem. 2000,96, 231-258. DOI: 10.1039/b002050h
10. R. J. Cox,* A. Sutherland, J. C. Vederas,* Bacterial Diaminopimelate Metabolism as a Target for Antibiotic Design, Bioorg. Med. Chem., 2000, 8, 843-871. DOI:10.1016/S0968-0896(00)00044-4
9. R. J. Cox,* Biosynthesis, Annu. Rep. Prog. Chem., Sect. B: Org. Chem. 1998,94, 187-206. DOI: 10.1039/oc094187
8. R. J. Cox,* The DAP Pathway to Lysine as a Target for Antimicrobial Agents, Nat. Prod. Rep. 1996, 13, 29-43. DOI: 10.1039/NP9961300029
7. R. J. Cox: Book Review, Introduction to Enzyme and Coenzyme Chemistry by T. D. H. Bugg, Blackwell, Oxford 2004, Nat Prod. Rep, 2005, 22, 127-127.
Book Chapters
6. R. J. Cox* and F. Glod, Fungal Secondary Metabolism in the Information Age , in Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine , Kluwer Press, 2004, New York, pp 69-81. ISBN-13: 9780306478666
5. R. J. Cox*, The Biosynthesis of polyketides, tetramic acids and pyridones in fungi, ACS Symposium Series on Polyketides: Biosynthesis, Biological Activity and Genetic Engineering , 2007, 955, chapter 3, 33-47. ISBN13: 9780841239784
4. R. J. Cox*, The Biosynthesis of Polyketides, Acyl Tetramic Acids and Pyridones by Filamentous Fungi, British Mycological Society Symposium series Exploitation of Fungi, Cambridge University Press, 2007, chapter 3, 25-44. ISBN-13:9780521859356
Electronic Publications
3. R. J. Cox* Reductive Hydrazination of a-ketoacid; Hydrazine carboxylic acid , Synthetic Page 192, 2002 : http://www.syntheticpages.org/pages/192
2. R. J. Cox* Epoxidation of an a-keto ester; Epoxide , Synthetic Page 194, 2002 : http://www.syntheticpages.org/pages/194
1. R. J. Cox* Formation of N-Acyl Phosphoramidates; Phosphoramidate , Synthetic Page 201, 2002 : http://www.syntheticpages.org/pages/201