Craig Armstrong's Research Page

Current work

Scheme for a 2-component switching system

Scheme for a 2-component switching system

My current research is focussed on the design and characterisation of conformational switches. This comprises work on the ZiCo switching system pioneered in this lab (Cerasoli et al. 2005), and the design of so-called minimalist EF-hands in an attempt to dissect the determinants of calcium affinity, calcium-triggered conformational changes, and partner selection.

Sewitching peptides

design of a new generation of switching peptide. Sequences known to adopt coiled-coil and zinc-finger conformations are used to generate sequences that might be capable of adopting both folds. Coiled-coil design rules are used to create a partner peptide for the switch peptide.

Much of my design work is inspired by bioinformatic analyses (Armstrong et al. 2009), I also have experience of applying bioinformatic techniques to the prediction of coiled-coil oligomeric state (Rackham et al. 2010, Armstrong and Vincent et al. 2011 submitted).

I have a wealth of experience in the biophysical characterisation of peptides, and have used techniques including circular dichroism spectroscopy, UV/vis spectroscopy (Berezovskaya et al. 2010), nuclear magnetic resonance spectroscopy, isothermal titration calorimetry and analytical ultracentrifugation.