Jordan Fletcher's Research Page

Current Research

As part of a collaboration between the laboratories of Dek Woolfson and Paula Booth, my current work is directed towards the development of self-assembled synthetic cytoskeletons. Using a bottom-up approach, we are developing a set of independently folded peptide components that can be connected together in such a way as to produce highly ordered and regular 3D matrices that mimic many aspects of the natural cytoskeleton.

Previous Work

During my final undergraduate year, and throughout my PhD, I worked in the Laboratory of Dr Tony Hughes in the Department of Pharmacology at The University of Melbourne. Here, my work was focused on the development of small peptide mimetics of the Brain-Derived Neurotrophic Factor (BDNF) – a protein which has long been touted as possessing clinical potential for the treatment of a range of neurodegenerative conditions. Through this work I was able to generate a range of highly potent, proteolytically stable, and membrane-permeable cyclic pentapeptides.

After my PhD I worked as a Post Doc funded by a Circadian Technologies Ltd under the supervision of Prof. Mibel Aguilar, and Prof. Patrick Perlmutter in the Department of Biochemistry, Monash University. Here, my work was focused on the design and synthesis of proteolytically stable T-cell epitopes for the treatment of cancer.

Selected Publications

01

Modified low molecular weight cyclic peptides as mimetics of BDNF with improved potency, proteolytic stability and transmembrane passage in vitro.

Fletcher J. M., & Hughes R. A

Bioorg. Med. Chem. 17, 2695-2702, (2009)

02

Interactions of HIV-1 Antibodies 2F5 and 4E10 with a gp41 Epitope Prebound to Host and Viral Membrane Model Systems.

Veiga A. S., Pattenden L. K., Fletcher J. M., Castanho M. A. R. B., & Aguilar, M. I.

ChemBioChem 10, 2695-2702, (2009)

03

Synthesis of Stapled β3-Peptides through Ring-Closing Metathesis.

Bergman Y. E., Del Borgo M. P., Gopalan, R. D., Jalal S., Unabia S. E., Ciampini M., Clayton D. J., Fletcher J. M., Mulder R. J., Wilce J. A., Aguilar M. I. & Perlmutter P.

Org. Lett. 11, 4438-4440, (2009)

04

Design of a Conformationally Defined and Proteolytically Stable Circular Mimetic of Brain-derived Neurotrophic Factor.

Fletcher J. M., Morton C. J., Zwar R. A., Murray S. S., O'Leary P. D., & Hughes R. A.

J Biol. Chem. 283, 33375-33383, (2008)

05

Novel monocyclic and bicyclic loop mimetics of brain-derived neurotrophic factor.

Fletcher J. M., & Hughes R. A.

J Pep Sci. 12, 515-524, (2006)

06

Design of conformationally-constrained peptides as mimetics of cellular growth factors.

Hughes R. A., Scanlon, D., Shabanpoor F., Fletcher J. M., Del Borgo M. P., O'Leary, P. D.

Peptide Science. 43, 217-218, (2006)

07

A novel approach to the regioselective synthesis of a disulfide-linked heterodimeric bicyclic peptide mimetic of brain-derived neurotrophic factor.

Fletcher J. M., & Hughes R. A.

Tetrahedron Lett. 45, 6999-7001, (2004)