Max Ryadnov's Research Page

Biomolecular engineering and design.

De novo biomolecular design with an emphasis on engineering new therapeutics and functional nanostructured materials is the major focus of research.

Hierarchical self-assembly of biomolecules is becoming an increasingly important tool for engineering new mesoscopic materials for biotechnology and medicine. Such materials are in great demand for a diverse range of applications, e.g. new antimicrobials, non-viral vectors for gene therapy, artificial matrices for tissue engineering and the like. All this is intimately associated with macromolecular design, which implies a search for new supramolecular arrangements and efficient routes of their production. One particular interest here is to broaden the current understanding of structural organization of biomolecules to apply that to designing various self-assembling systems possessing desired and predetermined properties to be further developed as:

Synthetic extracellular matrices. Over the last decade the development of supportive biocompatible molecular devices has emerged as a highly attractive approach in tissue regeneration without the limitations of current therapies. With this in mind, novel biodegradable and functionalizable peptide-based scaffolds are being developed as artificial extracellular matrices.

For example, synthetic matrices shown below are non-covalent self-arrangements of peptide-based nanofibres preprogrammed by specialist peptides designed to shape (left), interconnect (centre) and decorate (by recruiting gold nanoparticles labelled proteins, right) the nanofibres.

Some synthetic matrices showing non-covalent arrangements of peptid-based nanofibres

Non-viral vectors. Molecular therapy is a relatively new yet quickly growing field. It targets an infected or incorrectly working cell and has emerged as a promising alternative to common therapies. Cell targeting is achieved at subcellular or nano level. It is not surprising thus that natural nanosized self-assemblies, such as viruses, have quickly become a major tool for that. However, the use of viral vectors is often accompanied by inflammatory and immune responses. In this vein, main efforts involve the design of self-assembling virus-like systems as non-viral vectors.

Publications

21

Self-assembled templates for polypeptide synthesis.

Ryadnov, M. G. & Woolfson, D. N.

J. Am. Chem. Soc. 129 14074-14081 (2007)

20

The leucine-zipper as a building block for self-assembled protein fibers.

Ryadnov, M.G., Papapostolou, D. & Woolfson, D.N.

Methods Mol. Biol., in press (2007)

19

Engineering nanoscale order into a designed protein fiber.

Papapostolou, D., Smith, A. M., Atkins, E. D. T., Oliver, S. J., Ryadnov, M.G., Serpell, L. C. & Woolfson, D.N.

Proc Natl Acad Sci USA, 104 10853-10858 (2007)

18

Peptide α-helices for synthetic nanostructures.

Ryadnov, M.G.

Biochem Soc Trans. 35 487-491 (2007)

17

Self-assembling nanostructures from coiled coil peptides.

Ryadnov, M.G. & Woolfson, D.N.

In Nanobiotechnology II. More Concepts and Applications. (2007) (Mirkin, C. A. & Niemeyer, C. M. eds.) Wiley-VCH, Weinheim, pp 17-38

16

A self-assembling peptide polynanoreactor.

Ryadnov, M.G.

Angew. Chem.- Int. Ed. 46 969-972 (2007)

15

Peptide-based fibrous biomaterials: some things old, new and borrowed.

Woolfson, D.N. & Ryadnov, M.G.

Curr. Opin. Chem. Biol. 10 559-567 (2006)

14

MaP peptides: programming the self-assembly of peptide-based mesoscopic matrices.

Ryadnov, M.G. & Woolfson, D.N.

J. Am. Chem. Soc. 127 12407-12415 (2005)

13

Polar assembly in a designed protein fiber.

Smith, A.M., Acquah, S.F.A., Bone, N., Kroto, H.W., Ryadnov, M. G., Stevens, M.S.P., Walton, D.R.M. & Woolfson, D.N.

Angew. Chem.- Int. Ed. 44 325-328 (2005)

12

Fiber Recruiting Peptides: Noncovalent Decoration of an Engineered Protein Scaffold.

Ryadnov, M.G. & Woolfson, D.N.

J. Am. Chem. Soc. 126 7454-7455 (2004)

11

Engineering the morphology of a self-assembling protein fibre.

Ryadnov, M.G. & Woolfson, D.N.

Nat. Mater. 2 329-332 (2003)

10

Antimicrobial peptides containing arginine.

Ryadnov, M.G., Degtyareva, O.V., Kashparov, I.A. & Mitin, Yu.V.

Biochemistry (Mosc) 68 857-861 (2003)

9

"Belt and Braces": a peptide-based linker system of de novo design.

Ryadnov, M.G., Ceyhan, B., Niemeyer, C.M. & Woolfson, D.N.

J. Am. Chem. Soc. 125 9388-9394 (2003)

8

Introducing branches into a self-assembling peptide fiber.

Ryadnov, M.G. & Woolfson, D.N.

Angew. Chem. - Int. Ed. 42 3021-3023 (2003)

7

A new synthetic all-D-peptide with high bacterial and low mammalian cytotoxicity.

Ryadnov, M.G., Degtyareva, O.V., Kashparov, I.A. & Mitin, Yu.V.

Peptides 23 1869-1871 (2002)

6

Block-“inverse” synthesis of antimicrobial peptide temporin F in solution.

Ryadnov, M.G. & Mitin Yu. V.

In Peptides 2000 (2001) (Martinez, J. & Fehrenz, J.-A. eds.). Paris: EDK Editions Medicales et Scientifiques 2, 267-268

5

Suppression of epimerization by cupric (II) salts in peptide synthesis using free amino acids as amino components.

Ryadnov, M.G., Klimenko, L.V. & Mitin, Y.V.

J. Pept. Res. 53 322-328 (1999)

4

Inverse peptide synthesis in solution using free amino acids as amino components.

Mitin, Y. V. & Ryadnov, M. G.

Prot. Pept. Lett. 6 87-90 (1999)

3

An effective water-free aprotic system for dissolving free amino acids.

Ryadnov, M.G., Klimenko, L.V. & Mitin, Y.V.

Bioorg. Khim. 25 283-288 (1999)

2

Racemization-free system for peptide fragment coupling.

Ryadnov, M.G. & Mitin, Y.V.

In Peptides 1998 (1999) (Bajusz, S. & Hudecz, F. eds.), Akadémiai Kiadó, Budapest, pp 238-239.

1

Synthesis of peptides using free amino acids: the effect of inorganic compounds on the solubility of amino acids in aprotic solvents.

Ryadnov, M.G., Kashparova, N.I., Kashparov, I.A. & Mitin, Y.V.

Bioorg. Khim. 24 357-360 (1998)