Enzyme Structure determination
Dr Matt Crump - E-mail matt.crump@bristol.ac.uk

Chemical shifts table A list of chemical shifts for reference as requested - will include in handout 1 in future years
(PDF)

Lecture 1 - Introduction to NMR for structure determination - full version uploaded - 7th December 2011 - contains some of my boardwork from the lecture
(PDF 4.4Mb)

Lecture 1 Handout A compressed version of the slides - personally I would annotate handouts or make separate notes rather than printing all of the actual lecture slides
(PDF 1.2Mb)

Lecture 1 Notes - Notes for lecture 1
(Powerpoint presentation 400K)

Additional Notes This explains in more detail and in a conversational way what happens at an atomic level when we put spins into a magnetic field. I would recommend reading this.
(Word Document 540kb)


Click here for an excellent web based NMR book by Joseph Hornak

Lecture 2 - Applications of 1D NMR and introduction of 2D NMR based assignment
(PDF - Updated 07-12-2011 - 3.0Mb)

Lecture 2 Handout Compressed version of the slides to save trees....
(PDF - Updated 07-12-2011 - 2.6Mb)

Lecture 3 - TOCSY, NOESY and sequential assignment of a protein
(Powerpoint presentation - available December 16th 2011 sorry its late!)

Lecture 4 - December 16th 2011 The generation of a Protein Structure plus 15N labeling for larger proteins
(Powerpoint presentation)

Lecture 5 - New assignment strategies based on 13C and 15N labeling.. Application to large proteins. SAR by NMR in drug discovery
(uploaded Dec 16th 2011)

Lecture 6 - Relaxation (Uploaded December 16th 2011

And finally..I found this guide by Gerhard Wider - it gives an excellent overview of much of the material we have been thorugh. This is downloadable as a pdf.
(PDF 284K)


Figure: Ribonuclease structure showing histidine probes